中国科学家发现睡眠稳态调控机制 作者： 发布时间：/9/5 15:03:38 中国科学院脑科学与智能技术卓越创新中心徐敏研究组发现，基底前脑谷氨酸能神经元通过腺苷调控睡眠稳态。相关论文发表在9月4日出版的《科学》杂志上。
使用新开发的遗传编码腺苷传感器，研究人员发现小鼠基底前脑（BF，控制睡眠和清醒的关键区域）中细胞外腺苷浓度的活动依赖性快速增加。尽管BF胆碱能和谷氨酸能神经元的活性均与腺苷浓度的变化相关，但这些神经元在生理激发频率下的光遗传激活表明，谷氨酸能神经元对腺苷增加的贡献更大。BF谷氨酸能神经元的选择性敲除小鼠表现出腺苷增加的减少和睡眠稳态调节的受损。因此，BF中细胞特异性神经活动可动态控制睡眠稳态。
据介绍，睡眠和清醒受包括腺苷在内多种因素的稳态调节。然而，目前尚不清楚睡眠-清醒周期背后的神经活动如何控制脑中腺苷的释放。
附：英文原文
Title: Regulation of sleep homeostasis mediator adenosine by basal forebrain glutamatergic neurons
Author: Wanling Peng, Zhaofa Wu, Kun Song, Siyu Zhang, Yulong Li, Min Xu
Issue Volume: /09/04
Abstract: Sleep and wakefulness are homeostatically regulated by a variety of factors, including adenosine. However, how neural activity underlying the sleep-wake cycle controls adenosine release in the brain remains unclear. Using a newly developed genetically encoded adenosine sensor, we found an activity-dependent rapid increase in the concentration of extracellular adenosine in mouse basal forebrain (BF), a critical region controlling sleep and wakefulness. Although the activity of both BF cholinergic and glutamatergic neurons correlated with changes in the concentration of adenosine, optogenetic activation of these neurons at physiological firing frequencies showed that glutamatergic neurons contributed much more to the adenosine increase. Mice with selective ablation of BF glutamatergic neurons exhibited a reduced adenosine increase and impaired sleep homeostasis regulation. Thus, cell type specific neural activity in the BF dynamically controls sleep homeostasis.
DOI: 10.1126/science.abb0556
Source:
期刊信息 Science：《科学》，创刊于1880年。隶属于美国科学促进会，最新IF：41.037官方网址：投稿链接：
